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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASIP1 Polyclonal antibody

RASIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26064-1-AP

Synonyms

Ras-interacting protein 1, Ras interacting protein 1, Rain

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using RASIP1 Polyclonal antibody (26064-1-AP)

    WB analysis of mouse lung using 26064-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RASIP1 Polyclonal antibody (26064-1-AP)

    WB analysis of NIH/3T3 using 26064-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using RASIP1 Polyclonal antibody (26064-1-AP)

    IHC staining of human heart using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using RASIP1 Polyclonal antibody (26064-1-AP)

    IHC staining of human heart using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using RASIP1 Polyclonal antibody (26064-1-AP)

    IHC staining of human lung using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using RASIP1 Polyclonal antibody (26064-1-AP)

    IHC staining of human lung using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RASIP1 Polyclonal antibody (26064-1-AP)

    IF Staining of HepG2 using 26064-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RASIP1 antibody (26064-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Tested Applications

Positive WB detected inmouse lung tissue, NIH/3T3 cells
Positive IHC detected inhuman heart tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26064-1-AP targets RASIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASIP1 fusion protein Ag22036 相同性解析による交差性が予測される生物種
Full Name Ras interacting protein 1
Calculated molecular weight 963 aa, 103 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC021860
Gene symbol RASIP1
Gene ID (NCBI) 54922
RRIDAB_2880359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RASIP1, also named as Ras-interacting protein 1 or Rain, is a 963 amino acid protein, which contains one dilute domain and one Ras-associating domian. RASIP1 is highly expressed in heart and detected at lower levels in placenta and pancreas. RASIP1 is required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. RASIP1 acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis.

Protocols

Product Specific Protocols
WB protocol for RASIP1 antibody 26064-1-APDownload protocol
IHC protocol for RASIP1 antibody 26064-1-APDownload protocol
IF protocol for RASIP1 antibody 26064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols