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RASSF4 Polyclonal antibody
RASSF4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 19118-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, NCI-H1299 cells, U2OS cells, MG-63 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
19118-1-AP targets RASSF4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RASSF4 fusion protein Ag5780 相同性解析による交差性が予測される生物種 |
| Full Name | Ras association (RalGDS/AF-6) domain family member 4 |
| Calculated molecular weight | 321 aa, 37 kDa |
| Observed molecular weight | 34-37 kDa |
| GenBank accession number | BC032593 |
| Gene symbol | RASSF4 |
| Gene ID (NCBI) | 83937 |
| RRID | AB_10596921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RASSF4, a member of the classical RASSF family of scaffold proteins, is associated with alveolar rhabdomyosarcoma (aRMS), an aggressive pediatric cancer of muscle histogenesis. In aRMS, RASSF4 is upregulated in human cell lines and tumors and is oncogenic, portending a worse clinical prognosis. RASSF4 has been reported to directly interact with and activate K‐Ras in a GTP‐dependent manner. Ectopic expression of RASSF4 induces 293T apoptosis in a Ras‐dependent manner and inhibits tumour cell growth.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RASSF4 antibody 19118-1-AP | Download protocol |
| IHC protocol for RASSF4 antibody 19118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med RASSF4 inhibits cell proliferation and increases drug sensitivity in colorectal cancer through YAP/Bcl-2 pathway.
| ||
Tumour Biol RASSF4 is downregulated in nonsmall cell lung cancer and inhibits cancer cell proliferation and invasion. |
