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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASSF7 Polyclonal antibody

RASSF7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21753-1-AP

Synonyms

C11orf13, HRAS1, HRC1, RASSF7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RASSF7 Polyclonal antibody (21753-1-AP)

    WB analysis using 21753-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RASSF7 Polyclonal antibody (21753-1-AP)

    WB analysis of HepG2 using 21753-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RASSF7 Polyclonal antibody (21753-1-AP)

    IHC staining of human lung cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RASSF7 Polyclonal antibody (21753-1-AP)

    IHC staining of human lung cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using RASSF7 Polyclonal antibody (21753-1-AP)

    IHC staining of human thyroid cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21753-1-AP (RASSF7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using RASSF7 Polyclonal antibody (21753-1-AP)

    IHC staining of human thyroid cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21753-1-AP (RASSF7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, HepG2 cells, PC-12 cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21753-1-AP targets RASSF7 in WB, IHC, ELISA applications and shows reactivity with human, Rat samples.

Tested Reactivity human, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASSF7 fusion protein Ag16123 相同性解析による交差性が予測される生物種
Full Name Ras association (RalGDS/AF-6) domain family (N-terminal) member 7
Calculated molecular weight 373 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC106921
Gene symbol RASSF7
Gene ID (NCBI) 8045
RRIDAB_10858387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RASSF7, previously known as HRC1 (HRAS1 cluster 1) and C11orf13, is a member of the N-terminal RASSF family. RASSF7 protein localises to the centrosome and plays a key role in mitosis. RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. RASSF7 has three isoforms with the MW 40 kDa, 36 kDa and 35 kDa.

Protocols

Product Specific Protocols
WB protocol for RASSF7 antibody 21753-1-APDownload protocol
IHC protocol for RASSF7 antibody 21753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols