RB1 Polyclonal antibody

Name: RB1 Polyclonal antibody
Brand: Proteintech
SKU: 25628-1-AP

RB1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 25628-1-AP

Synonyms

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1

Validation Data Gallery

Filter:

AllWBIPIF

WB analysis of Jurkat using 25628-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 25628-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25628-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 25628-1-AP
IP result of anti-RB1 (IP:25628-1-AP, 4ug; Detection:25628-1-AP 1:300) with Jurkat cells lysate 3400ug.
IF Staining of HepG2 using 25628-1-AP
Immunofluorescent analysis of HepG2 cells using 25628-1-AP (RB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 25628-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, A431 cells
Positive IP detected in	Jurkat cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

25628-1-AP targets RB1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RB1 fusion protein Ag22578 相同性解析による交差性が予測される生物種
Full Name	retinoblastoma 1
Calculated molecular weight	928 aa, 106 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC039060
Gene symbol	RB1
Gene ID (NCBI)	5925
RRID	AB_2880168
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RB1 antibody 25628-1-AP	Download protocol
IF protocol for RB1 antibody 25628-1-AP	Download protocol
IP protocol for RB1 antibody 25628-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Artif Cells Nanomed Biotechnol Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway. Authors - Tianqi Li View Article
human	WB	Oncol Rep Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma. Authors - Da-Peng Yun View Article
human	IF	Oncol Lett 14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells. Authors - Kun Lu View Article
human	WB	Cell Death Dis MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis. Authors - Fengxia Chen View Article
human	WB	Biomed Pharmacother Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo. Authors - Yudi Xiong View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RB1 Polyclonal antibody

RB1 Polyclonal Antibody for WB, IP, IF, ELISA

Validation Data Gallery

WB analysis of Jurkat using 25628-1-AP

WB analysis of A431 using 25628-1-AP

WB analysis of Jurkat using 25628-1-AP

IP experiment of Jurkat using 25628-1-AP

IF Staining of HepG2 using 25628-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.

Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.

Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.