RB1 Polyclonal antibody
RB1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 30373-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HT-1080 cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30373-1-AP targets RB1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | retinoblastoma 1 |
Calculated molecular weight | 928 aa, 106 kDa |
Observed molecular weight | 110 kDa |
GenBank accession number | BC039060 |
Gene symbol | RB1 |
Gene ID (NCBI) | 5925 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).
Protocols
Product Specific Protocols | |
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WB protocol for RB1 antibody 30373-1-AP | Download protocol |
IF protocol for RB1 antibody 30373-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |