RB1 Polyclonal antibody

RB1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30373-1-AP

Synonyms

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inK-562 cells, HT-1080 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30373-1-AP targets RB1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name retinoblastoma 1
Calculated molecular weight 928 aa, 106 kDa
Observed molecular weight110 kDa
GenBank accession numberBC039060
Gene symbol RB1
Gene ID (NCBI) 5925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).

Protocols

Product Specific Protocols
WB protocol for RB1 antibody 30373-1-APDownload protocol
IF protocol for RB1 antibody 30373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols