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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RB1 Polyclonal antibody

RB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30373-1-AP

Synonyms

pRb, pp110, p110-RB1 , p110-RB1, p105-Rb

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RB1 Polyclonal antibody (30373-1-AP)

    WB analysis using 30373-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30373-1-AP (RB1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RB1 Polyclonal antibody (30373-1-AP)

    IHC staining of human ovary cancer using 30373-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30373-1-AP (RB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RB1 Polyclonal antibody (30373-1-AP)

    IHC staining of human ovary cancer using 30373-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30373-1-AP (RB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RB1 Polyclonal antibody (30373-1-AP)

    IF Staining of HepG2 using 30373-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RB1 antibody (30373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inK-562 cells, HT-1080 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30373-1-AP targets RB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name retinoblastoma 1
Calculated molecular weight 928 aa, 106 kDa
Observed molecular weight110 kDa
GenBank accession numberBC039060
Gene symbol RB1
Gene ID (NCBI) 5925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).

Protocols

Product Specific Protocols
WB protocol for RB1 antibody 30373-1-APDownload protocol
IHC protocol for RB1 antibody 30373-1-APDownload protocol
IF protocol for RB1 antibody 30373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols