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  • Featured Product
  • KD/KO Validated

RB1 Monoclonal antibody

RB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A6

Cat no : 67521-1-Ig

Synonyms

pRb, pp110, p110-RB1 , p110-RB1, p105-Rb

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RB1 Monoclonal antibody (67521-1-Ig)

    WB analysis of Jurkat using 67521-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RB1 Monoclonal antibody (67521-1-Ig)

    WB analysis of K-562 using 67521-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RB1 Monoclonal antibody (67521-1-Ig)

    WB analysis of MCF-7 using 67521-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RB1 Monoclonal antibody (67521-1-Ig)

    WB analysis of Jurkat using 67521-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RB1 Monoclonal antibody (67521-1-Ig)

    WB analysis of K-562 using 67521-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using RB1 Monoclonal antibody (67521-1-Ig)

    WB analysis of HL-60 using 67521-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TF-1 cells using RB1 Monoclonal antibody (67521-1-Ig)

    WB analysis of TF-1 using 67521-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Insulinoma tissue using RB1 Monoclonal antibody (67521-1-Ig)

    IHC staining of Insulinoma using 67521-1-Ig

    Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Insulinoma tissue using RB1 Monoclonal antibody (67521-1-Ig)

    IHC staining of Insulinoma using 67521-1-Ig

    Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using RB1 Monoclonal antibody (67521-1-Ig)

    IF Staining of SH-SY5Y using 67521-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RB1 Monoclonal antibody (67521-1-Ig)

    IF Staining of U2OS using 67521-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, K-562 cells, MCF-7 cells, TF-1 cells
Positive IHC detected inInsulinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67521-1-Ig targets RB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RB1 fusion protein Ag22578 相同性解析による交差性が予測される生物種
Full Name retinoblastoma 1
Calculated molecular weight 928 aa, 106 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC039060
Gene symbol RB1
Gene ID (NCBI) 5925
RRIDAB_2882741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Protocols

Product Specific Protocols
WB protocol for RB1 antibody 67521-1-IgDownload protocol
IHC protocol for RB1 antibody 67521-1-IgDownload protocol
IF protocol for RB1 antibody 67521-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol

RB1 Mutations Induce Smoking-Related Bladder Cancer by Modulating the Cytochrome P450 Pathway

Authors - Zhenguang Mao
  • KD Validated
View Article
humanWB

Cancer Res

CDK4/6 Alters TBK1 Phosphorylation to Inhibit the STING Signaling Pathway in Prostate Cancer

Authors - Wei Li
View Article
humanWB

Life (Basel)

Investigating the Effects of Chelidonic Acid on Oxidative Stress-Induced Premature Cellular Senescence in Human Skin Fibroblast Cells

Authors - Burcu Turkoglu
View Article