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KD/KO Validated

RB1CC1 Polyclonal antibody

Name: RB1CC1 Polyclonal antibody
Brand: Proteintech
SKU: 10043-2-AP

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10043-2-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HeLa using 10043-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10043-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10043-2-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10043-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10043-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10043-2-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10043-2-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10043-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 10043-2-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10043-2-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 10043-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 10043-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10043-2-AP
Immunofluorescent analysis of Hela cells, using RB1CC1 antibody 10043-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 10043-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, mouse brain tissue, MCF-7 cells
Positive IHC detected in	human breast cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 17 publications below
IHC	See 3 publications below
IF	See 7 publications below
IP	See 1 publications below

Product Information

10043-2-AP targets RB1CC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein 相同性解析による交差性が予測される生物種
Full Name	RB1-inducible coiled-coil 1
Calculated molecular weight	1594 aa, 183 kDa
Observed molecular weight	180-200 kDa
GenBank accession number	BC017556
Gene symbol	RB1CC1
Gene ID (NCBI)	9821
RRID	AB_2253571
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation[PMID:15968549 ]. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation [PMID: 19258318]. It is probably involved in the tumorigenesis of breast cancer[PMID: 14533007]. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene[PMID: 21602932]. The immunogen of this antibody is C-terminal 154aa of RB1CC1.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 10043-2-AP	Download protocol
IHC protocol for RB1CC1 antibody 10043-2-AP	Download protocol
IF protocol for RB1CC1 antibody 10043-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Noncanonical autophagy promotes the visual cycle. Authors - Kim Ji-Young JY KD Validated View Article
human	WB	Nat Immunol Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms. Authors - Michal P Wandel KD Validated View Article
human	WB	Nat Cell Biol mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6. Authors - Nazio Francesca F View Article
human	IF	Nat Cell Biol ATM functions at the peroxisome to induce pexophagy in response to ROS. Authors - Jiangwei Zhang View Article
mouse	WB	Genes Dev p62/SQSTM1 synergizes with autophagy for tumor growth in vivo. Authors - Huijun Wei View Article
human, mouse	WB, IF	Nat Struct Mol Biol Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy. Authors - Noor Gammoh View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 10043-2-AP

WB analysis of HeLa using 10043-2-AP

WB analysis of Jurkat using 10043-2-AP

WB analysis of HeLa using 10043-2-AP

WB analysis of HeLa using 10043-2-AP

WB analysis of MCF-7 using 10043-2-AP

WB analysis of mouse brain using 10043-2-AP

IHC staining of human breast cancer using 10043-2-AP

IHC staining of human testis using 10043-2-AP

IHC staining of human testis using 10043-2-AP

IHC staining of human breast cancer using 10043-2-AP

IHC staining of human breast cancer using 10043-2-AP

IF Staining of HeLa using 10043-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Noncanonical autophagy promotes the visual cycle.

Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.

mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6.

ATM functions at the peroxisome to induce pexophagy in response to ROS.

p62/SQSTM1 synergizes with autophagy for tumor growth in vivo.

Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy.

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