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  • Featured Product
  • KD/KO Validated

RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10069-1-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of HEK-293 using 10069-1-AP

    WB result of RB1CC1 antibody (10069-1-AP, 1:1000) with si-Control and si-RB1CC1 transfected HEK293 cells.

  • Western Blot (WB) analysis of MCF-7 cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of MCF-7 using 10069-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of mouse skeletal muscle using 10069-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of HeLa using 10069-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of MCF-7 using 10069-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of MCF-7 using 10069-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of MCF-7 using 10069-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of HeLa using 10069-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of HeLa using 10069-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of HeLa using 10069-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of HeLa using 10069-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of HeLa using 10069-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of NIH/3T3 using 10069-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    WB analysis of NIH/3T3 using 10069-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RB1CC1 Polyclonal antibody (10069-1-AP)

    IHC staining of human breast cancer using 10069-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10069-1-AP (RB1CC1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using RB1CC1 Polyclonal antibody (10069-1-AP)

    IHC staining of human appendicitis using 10069-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RB1CC1 Polyclonal antibody (10069-1-AP)

    IHC staining of human kidney using 10069-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10069-1-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RB1CC1 Polyclonal antibody (10069-1-AP)

    IF Staining of HeLa using 10069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RB1CC1 antibody (10069-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inMCF7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, mouse skeletal muscle tissue
Positive IHC detected inhuman appendicitis tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10069-1-AP targets RB1CC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RB1CC1 fusion protein Ag0003 相同性解析による交差性が予測される生物種
Full Name RB1-inducible coiled-coil 1
Calculated molecular weight 1594 aa, 183 kDa
Observed molecular weight 183 kDa
GenBank accession numberBC017556
Gene symbol RB1CC1
Gene ID (NCBI) 9821
RRIDAB_2877722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 10069-1-APDownload protocol
IHC protocol for RB1CC1 antibody 10069-1-APDownload protocol
IF protocol for RB1CC1 antibody 10069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

26S proteasomes become stably activated upon heat shock when ubiquitination and protein degradation increase.

Authors - Donghoon Lee
View Article
humanIF

Dev Cell

The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A.

Authors - Claudia Puri
View Article