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  • Featured Product
  • KD/KO Validated

RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21269-1-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RB1CC1 Polyclonal antibody (21269-1-AP)

    WB analysis of HEK-293 using 21269-1-AP

    WB result of RB1CC1 antibody (21269-1-AP, 1:1000) with si-Control and si-RB1CC1 transfected HEK293 cells.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (21269-1-AP)

    WB analysis of HeLa using 21269-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RB1CC1 Polyclonal antibody (21269-1-AP)

    WB analysis of HeLa using 21269-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RB1CC1 Polyclonal antibody (21269-1-AP)

    WB analysis of HEK-293 using 21269-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RB1CC1 Polyclonal antibody (21269-1-AP)

    WB analysis of MCF-7 using 21269-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RB1CC1 Polyclonal antibody (21269-1-AP)

    WB analysis of HEK-293 using 21269-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RB1CC1 Polyclonal antibody (21269-1-AP)

    IHC staining of human ovary cancer using 21269-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21269-1-AP (RB1CC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21269-1-AP targets RB1CC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RB1CC1 fusion protein Ag15739 相同性解析による交差性が予測される生物種
Full Name RB1-inducible coiled-coil 1
Calculated molecular weight 1594 aa, 183 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC017556
Gene symbol RB1CC1
Gene ID (NCBI) 9821
RRIDAB_10733100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RB1CC1.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 21269-1-APDownload protocol
IHC protocol for RB1CC1 antibody 21269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols