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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RB1CC1 Monoclonal antibody

RB1CC1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8C8

Cat no : 68564-1-Ig

Synonyms

CC1, DRAGOU14, FIP200, KIAA0203, RB1 inducible coiled coil 1, RB1CC1, RBICC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RB1CC1 Monoclonal antibody (68564-1-Ig)

    WB analysis using 68564-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68564-1-Ig (RB1CC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RB1CC1 Monoclonal antibody (68564-1-Ig)

    WB analysis using 68564-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68564-1-Ig (RB1CC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68564-1-Ig targets RB1CC1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RB1CC1 fusion protein Ag10508 相同性解析による交差性が予測される生物種
Full Name RB1-inducible coiled-coil 1
Calculated molecular weight 1594 aa, 183 kDa
Observed molecular weight200 kDa
GenBank accession numberBC017556
Gene symbol RB1CC1
Gene ID (NCBI) 9821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 68564-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols