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  • Featured Product
  • KD/KO Validated

RBAP48 Monoclonal antibody, PBS Only

RBAP48 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C4D6

Cat no : 66060-1-PBS

Synonyms

RBBP4, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48, CAF-I 48 kDa subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBAP48 Monoclonal antibody (66060-1-Ig)

    WB analysis using 66060-1-Ig (same clone as 66060-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RBAP48 Monoclonal antibody (66060-1-Ig)

    WB analysis of HeLa using 66060-1-Ig (same clone as 66060-1-PBS)

    WB result of RBAP48 antibody (66060-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells. This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RBAP48 Monoclonal antibody (66060-1-Ig)

    WB analysis of HeLa using 66060-1-Ig (same clone as 66060-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBAP48 Monoclonal antibody (66060-1-Ig)

    IHC staining of human cervical cancer using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBAP48 Monoclonal antibody (66060-1-Ig)

    IHC staining of human cervical cancer using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RBAP48 Monoclonal antibody (66060-1-Ig)

    IHC staining of human testis using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66060-1-Ig(RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RBAP48 Monoclonal antibody (66060-1-Ig)

    IHC staining of human testis using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66060-1-Ig(RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBAP48 Monoclonal antibody (66060-1-Ig)

    IF Staining of HepG2 using 66060-1-Ig (same clone as 66060-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66060-1-Ig (RBAP48 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RBAP48 Monoclonal antibody (66060-1-Ig)

    FC experiment of HepG2 using 66060-1-Ig (same clone as 66060-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human RBAP48 (66060-1-Ig, Clone:5C4D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66060-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66060-1-PBS targets RBAP48 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RBAP48 fusion protein Ag6196 相同性解析による交差性が予測される生物種
Full Name retinoblastoma binding protein 4
Calculated molecular weight 48 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC053904
Gene symbol RBBP4
Gene ID (NCBI) 5928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).