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RBAP48 Polyclonal antibody

Name: RBAP48 Polyclonal antibody
Brand: Proteintech
SKU: 20364-1-AP
Rating: 5.00 (1 reviews)

RBAP48 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 20364-1-AP

Synonyms

RBBP4, NURF55, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 20364-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20364-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20364-1-AP
WB result of RBAP48 antibody (20364-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.
WB analysis of HepG2 using 20364-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20364-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20364-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20364-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 20364-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20364-1-AP
IP result of anti-RBAP48 (IP:20364-1-AP, 4ug; Detection:20364-1-AP 1:1000) with HeLa cells lysate 2000ug.
IHC staining of human cervical cancer using 20364-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 20364-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 20364-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 20364-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 20364-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 20364-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 20364-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBAP48 antibody (20364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 20364-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, L02 cells, Jurkat cells, HepG2 cells, K-562 cells, HeLa cells, human brain tissue, HEK-293 cells, SH-SY5Y cells, mouse brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human cervical cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 13 publications below
IHC	See 2 publications below
IP	See 2 publications below

Product Information

20364-1-AP targets RBAP48 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	retinoblastoma binding protein 4
Calculated molecular weight	48 kDa
Observed molecular weight	48-50 kDa
GenBank accession number	NM_005610
Gene symbol	RBBP4
Gene ID (NCBI)	5928
RRID	AB_10694151
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.

Protocols

Product Specific Protocols
WB protocol for RBAP48 antibody 20364-1-AP	Download protocol
IHC protocol for RBAP48 antibody 20364-1-AP	Download protocol
IF protocol for RBAP48 antibody 20364-1-AP	Download protocol
IP protocol for RBAP48 antibody 20364-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. Authors - Pingping Zhu View Article
human	WB, IHC	J Clin Invest ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells. Authors - Pingping Zhu View Article
human	WB	Nat Commun Identification of proximal SUMO-dependent interactors using SUMO-ID. Authors - Orhi Barroso-Gomila View Article
	WB	Carcinogenesis circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression. Authors - Wei Zhan View Article
human,mouse	WB	Int J Biol Sci Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism. Authors - Shang Zhi KD Validated View Article
human	WB	Chembiochem Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells. Authors - Fang-Fang Zhuo KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Katherine (Verified Customer) (09-26-2023)

Nice band

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: 293T

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBAP48 Polyclonal antibody

RBAP48 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 20364-1-AP

WB analysis of Jurkat using 20364-1-AP

WB analysis of HeLa using 20364-1-AP

WB analysis of HepG2 using 20364-1-AP

WB analysis of K-562 using 20364-1-AP

WB analysis of HeLa using 20364-1-AP

WB analysis of human brain using 20364-1-AP

WB analysis of L02 using 20364-1-AP

IP experiment of HeLa using 20364-1-AP

IHC staining of human cervical cancer using 20364-1-AP

IHC staining of human cervical cancer using 20364-1-AP

IHC staining of human cervical cancer using 20364-1-AP

IHC staining of human cervical cancer using 20364-1-AP

IHC staining of human breast cancer using 20364-1-AP

IHC staining of human breast cancer using 20364-1-AP

IF Staining of HeLa using 20364-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.

Identification of proximal SUMO-dependent interactors using SUMO-ID.

circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression.

Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.

Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells.

Reviews