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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RbAp46 Recombinant antibody (84330-1-RR)

    WB analysis using 84330-1-RR (same clone as 84330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84330-1-RR (RBBP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using RbAp46 Recombinant antibody (84330-1-RR)

    IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using RbAp46 Recombinant antibody (84330-1-RR)

    IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RbAp46 Recombinant antibody (84330-1-RR)

    IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RbAp46 Recombinant antibody (84330-1-RR)

    IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RbAp46 Recombinant antibody (84330-1-RR)

    IF Staining of A431 using 84330-1-RR (same clone as 84330-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBBP7 antibody (84330-1-RR, Clone: 241189D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RbAp46 Recombinant antibody (84330-1-RR)

    FC experiment of HeLa using 84330-1-RR (same clone as 84330-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RBBP7 Recombinant antibody (84330-1-RR, Clone:241189D6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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RbAp46 Recombinant antibody, PBS Only

RbAp46 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84330-1-PBS
Clone No.241189D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

RBBP7, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46, Histone-binding protein RBBP7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84330-1-PBS targets RbAp46 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name retinoblastoma binding protein 7
Calculated molecular weight 48 kDa
GenBank accession numberNM_002893
Gene Symbol RBBP7
Gene ID (NCBI) 5931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16576
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.