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- KD/KO Validated
RBBP8 Polyclonal antibody
RBBP8 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 12624-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
12624-1-AP targets RBBP8 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RBBP8 fusion protein Ag3314 相同性解析による交差性が予測される生物種 |
| Full Name | retinoblastoma binding protein 8 |
| Calculated molecular weight | 103 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC030590 |
| Gene symbol | RBBP8 |
| Gene ID (NCBI) | 5932 |
| RRID | AB_2175250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBBP8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. It functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It interacted with BRCA1 to form a complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Also it promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBBP8 antibody 12624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.
| ||
J Biol Chem PTIP UFMylation promotes replication fork degradation in BRCA1-deficient cells | ||
Cell Biochem Funct The CTIP-mediated repair of TNF-α-induced DNA double-strand break was impaired by miR-130b in cervical cancer cell. | ||
Cell Biosci Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair |
