Validation Data Gallery
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Tested Applications
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
26367-1-AP targets RBCK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RBCK1 fusion protein Ag24405 相同性解析による交差性が予測される生物種 |
| Full Name | RanBP-type and C3HC4-type zinc finger containing 1 |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC015219 |
| Gene Symbol | RBCK1 |
| Gene ID (NCBI) | 10616 |
| RRID | AB_2880493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| IP protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis RBCK1 regulates the progression of ER-positive breast cancer through the HIF1α signaling | ||
Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis
| ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma | ||
Hum Cell Regulation of PTEN and ovarian cancer progression by an E3 ubiquitin ligase RBCK1.
| ||
Biochem Biophys Res Commun Mechanistic insights into the homo-dimerization of HOIL-1L and SHARPIN | ||
BMC Musculoskelet Disord Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China. |