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  • KD/KO Validated

RBCK1 Polyclonal antibody

RBCK1 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26367-1-AP

Synonyms

C20orf18, HBV associated factor 4, HOIL 1, HOIL1, RBCK1, RBCK2, RING finger protein 54, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4


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Tested Applications

Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26367-1-AP is RBCK1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBCK1 fusion protein Ag24405 相同性解析による交差性が予測される生物種
Full Name RanBP-type and C3HC4-type zinc finger containing 1
Calculated molecular weight 58 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC015219
Gene symbol RBCK1
Gene ID (NCBI) 10616
RRIDAB_2880493
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for RBCK1 antibody 26367-1-APDownload protocol
IP protocol for RBCK1 antibody 26367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

Cell Death Dis

RBCK1 regulates the progression of ER-positive breast cancer through the HIF1α signaling

Authors - Zhiguo Niu
humanWB,IHC

Oncogene

The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis

Authors - Yapeng Wang
  • KD Validated
humanIHC,WB

Front Immunol

Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma

Authors - Liangmin Fu
humanIHC

Hum Cell

Regulation of PTEN and ovarian cancer progression by an E3 ubiquitin ligase RBCK1.

Authors - Guanghui Wang
  • KD Validated
humanWB

Biochem Biophys Res Commun

Mechanistic insights into the homo-dimerization of HOIL-1L and SHARPIN

Authors - Yan Zhang
humanWB

BMC Musculoskelet Disord

Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China.

Authors - Lin Chen