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- KD/KO Validated
RBL2 Polyclonal antibody
RBL2 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 27251-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
Product Information
27251-1-AP targets RBL2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RBL2 fusion protein Ag25087 相同性解析による交差性が予測される生物種 |
| Full Name | retinoblastoma-like 2 (p130) |
| Calculated molecular weight | 130 kDa |
| Observed molecular weight | 128 kDa |
| GenBank accession number | BC034490 |
| Gene symbol | RBL2 |
| Gene ID (NCBI) | 5934 |
| RRID | AB_2880819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBL2, also named as Retinoblastoma-like protein 2 or p130, is a 1130 amino acid protein, which Belongs to the retinoblastoma protein (RB) family. RBL2 is a Key regulator of entry into cell division and is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. RBL2 probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 may act as a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBL2 antibody 27251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms. | ||
Mol Pharm Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells. | ||
Life Sci Early-senescent bone marrow mesenchymal stem cells promote C2C12 cell myogenic differentiation by preventing the nuclear translocation of FOXO3. | ||
Nat Commun An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis
| ||
