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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM10 Recombinant antibody, PBS Only (Detector)

RBM10 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241151D7

Cat no : 84104-2-PBS

Synonyms

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM10 Recombinant antibody (84104-2-RR)

    WB analysis using 84104-2-RR (same clone as 84104-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-2-RR (RBM10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84104-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    WB analysis of rat brain using 84104-2-RR (same clone as 84104-2-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84104-2-RR (RBM10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84104-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    IHC staining of mouse brain using 84104-2-RR (same clone as 84104-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    IHC staining of mouse brain using 84104-2-RR (same clone as 84104-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    IHC staining of mouse brain using 84104-2-RR (same clone as 84104-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBM10 Recombinant antibody (84104-2-RR)

    IF Staining of A431 using 84104-2-RR (same clone as 84104-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-2-RR, Clone: 241151D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84104-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using RBM10 Recombinant antibody (84104-2-RR)

    FC experiment of A431 using 84104-2-RR (same clone as 84104-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RBM10 Recombinant antibody (84104-2-RR, Clone:241151D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84104-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01041-2

    Cytometric bead array standard curve of MP01041-2

    Cytometric bead array standard curve of MP01041-2, RBM10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84104-1-PBS. Detection antibody: 84104-2-PBS. Standard: Ag5788. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84104-2-PBS targets RBM10 as part of a matched antibody pair:

MP01041-2: 84104-1-PBS capture and 84104-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM10 fusion protein Ag5788 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 10
Calculated molecular weight 104 kDa
Observed molecular weight100-120 kDa
GenBank accession numberBC024153
Gene symbol RBM10
Gene ID (NCBI) 8241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .