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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM10 Recombinant antibody (84104-4-RR)

    WB analysis using 84104-4-RR (same clone as 84104-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-4-RR (RBM10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBM10 Recombinant antibody (84104-4-RR)

    IF Staining of A431 using 84104-4-RR (same clone as 84104-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-4-RR, Clone: 241151G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84104-4-RR

    Affinity and Kinetic Characterization of 84104-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84104-4-RR against Human RBM10 were performed. The affinity constant is 0.33 nM.

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RBM10 Recombinant antibody, PBS Only

RBM10 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84104-4-PBS
Clone No.241151G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84104-4-PBS targets RBM10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM10 fusion protein Ag5788 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 10
Calculated molecular weight 104 kDa
Observed molecular weight100-120 kDa
GenBank accession numberBC024153
Gene Symbol RBM10
Gene ID (NCBI) 8241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP98175
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .