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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM12B Polyclonal antibody

RBM12B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17137-1-AP

Synonyms

RBM12B, RNA binding motif protein 12B, RNA binding protein 12B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM12B Polyclonal antibody (17137-1-AP)

    WB analysis using 17137-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17137-1-AP (RBM12B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using RBM12B Polyclonal antibody (17137-1-AP)

    IHC staining of human liver using 17137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17137-1-AP (RBM12B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using RBM12B Polyclonal antibody (17137-1-AP)

    IHC staining of human liver using 17137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17137-1-AP (RBM12B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBM12B Polyclonal antibody (17137-1-AP)

    IF Staining of A431 using 17137-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM12B antibody (17137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17137-1-AP targets RBM12B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM12B fusion protein Ag10894 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 12B
Calculated molecular weight 765 aa, 87 kDa, 118 kDa
Observed molecular weight118-130 kDa
GenBank accession numberBC039260
Gene symbol RBM12B
Gene ID (NCBI) 389677
RRIDAB_2175746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RBM12B antibody 17137-1-APDownload protocol
IHC protocol for RBM12B antibody 17137-1-APDownload protocol
IF protocol for RBM12B antibody 17137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Stress granules regulate stress-induced paraspeckle assembly.

Authors - Haiyan An
View Article