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KD/KO Validated

RBM15B Polyclonal antibody

Name: RBM15B Polyclonal antibody
Brand: Proteintech
SKU: 22249-1-AP

RBM15B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22249-1-AP

Synonyms

HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B

Validation Data Gallery

Filter:

AllWBIP

WB analysis of HeLa using 22249-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 22249-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22249-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22249-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 22249-1-AP
IP result of anti-RBM15B (IP:22249-1-AP, 4ug; Detection:22249-1-AP 1:500) with HeLa cells lysate 2500ug.

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Cat No. 22249-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, mouse brain tissue
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IHC	See 4 publications below
CoIP	See 1 publications below
RIP	See 2 publications below

Product Information

The immunogen of 22249-1-AP is RBM15B Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RBM15B fusion protein Ag17744 相同性解析による交差性が予測される生物種
Full Name	RNA binding motif protein 15B
Calculated molecular weight	890 aa, 97 kDa
Observed molecular weight	100-120 kDa, 70 kDa
GenBank accession number	BC001367
Gene symbol	RBM15B
Gene ID (NCBI)	29890
RRID	AB_2879048
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa), thus this antibody can recognize full length of RBM15B(~100kDa) and splice-one(70kDa)

Protocols

Product Specific Protocols
WB protocol for RBM15B antibody 22249-1-AP	Download protocol
IP protocol for RBM15B antibody 22249-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature METTL3 regulates heterochromatin in mouse embryonic stem cells. Authors - Wenqi Xu KO Validated View Article
human	WB	Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. Authors - Elodie Villa View Article
human	RIP	Elife SNORD90 induces glutamatergic signaling following treatment with monoaminergic antidepressants Authors - Rixing Lin View Article
human	WB	eNeuro Altered Expression of the m6A Methyltransferase METTL3 in Alzheimer's Disease. Authors - He Huang View Article
human	IHC	Front Genet m6A Regulatory Gene-Mediated Methylation Modification in Glioma Survival Prediction. Authors - Guiyun Zhang View Article
human	IHC	Med Sci Monit Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma. Authors - Yingying Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM15B Polyclonal antibody

RBM15B Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 22249-1-AP

WB analysis of mouse brain using 22249-1-AP

WB analysis of HeLa using 22249-1-AP

WB analysis of HEK-293 using 22249-1-AP

IP experiment of HeLa using 22249-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

METTL3 regulates heterochromatin in mouse embryonic stem cells.

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

SNORD90 induces glutamatergic signaling following treatment with monoaminergic antidepressants

Altered Expression of the m6A Methyltransferase METTL3 in Alzheimer's Disease.

m6A Regulatory Gene-Mediated Methylation Modification in Glioma Survival Prediction.

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.