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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM15B Monoclonal antibody, PBS Only

RBM15B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2C11

Cat no : 67506-1-PBS

Synonyms

HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis of K-562 using 67506-1-Ig (same clone as 67506-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis of COLO 320 using 67506-1-Ig (same clone as 67506-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67506-1-PBS targets RBM15B in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBM15B fusion protein Ag17853 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 15B
Calculated molecular weight 890 aa, 97 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC001367
Gene symbol RBM15B
Gene ID (NCBI) 29890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).