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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM17 Polyclonal antibody

RBM17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13918-1-AP

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM17 Polyclonal antibody (13918-1-AP)

    WB analysis using 13918-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13918-1-AP (RBM17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RBM17 Polyclonal antibody (13918-1-AP)

    IP experiment of MCF-7 using 13918-1-AP

    IP result of anti-RBM17 (IP:13918-1-AP, 4ug; Detection:13918-1-AP 1:10000) with MCF-7 cells lysate 1000 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RBM17 Polyclonal antibody (13918-1-AP)

    IHC staining of human ovary cancer using 13918-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13918-1-AP (RBM17 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM17 Polyclonal antibody (13918-1-AP)

    IF Staining of HeLa using 13918-1-AP

    Immunofluorescent analysis of Hela cells, using RBM17 antibody 13918-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inBxPC-3 cells, A2780 cells, MCF-7 cells, HeLa cells, PC-3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13918-1-AP targets RBM17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM17 fusion protein Ag4921 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 17
Calculated molecular weight 401 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC039322
Gene symbol RBM17
Gene ID (NCBI) 84991
RRIDAB_2238079
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

Protocols

Product Specific Protocols
WB protocol for RBM17 antibody 13918-1-APDownload protocol
IHC protocol for RBM17 antibody 13918-1-APDownload protocol
IF protocol for RBM17 antibody 13918-1-APDownload protocol
IP protocol for RBM17 antibody 13918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.

Authors - Sean-Patrick Riechers
View Article
humanWB

J Exp Clin Cancer Res

CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.

Authors - Dongni Chen
View Article