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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM17 Polyclonal antibody, PBS Only

RBM17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13918-1-PBS

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM17 Polyclonal antibody (13918-1-AP)

    WB analysis using 13918-1-AP (same clone as 13918-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13918-1-AP (RBM17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RBM17 Polyclonal antibody (13918-1-AP)

    IP experiment of MCF-7 using 13918-1-AP (same clone as 13918-1-PBS)

    IP result of anti-RBM17 (IP:13918-1-AP, 4ug; Detection:13918-1-AP 1:10000) with MCF-7 cells lysate 1000 ug. This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RBM17 Polyclonal antibody (13918-1-AP)

    IHC staining of human ovary cancer using 13918-1-AP (same clone as 13918-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13918-1-AP (RBM17 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM17 Polyclonal antibody (13918-1-AP)

    IF Staining of HeLa using 13918-1-AP (same clone as 13918-1-PBS)

    Immunofluorescent analysis of Hela cells, using RBM17 antibody 13918-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13918-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13918-1-PBS targets RBM17 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM17 fusion protein Ag4921 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 17
Calculated molecular weight 401 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC039322
Gene symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.