Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, mouse thymus tissue, rat thymus tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human ovary cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15374-1-AP targets RBM17 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RBM17 fusion protein Ag0985 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 17 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC007871 |
| Gene Symbol | RBM17 |
| Gene ID (NCBI) | 84991 |
| RRID | AB_2175914 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96I25 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM17 antibody 15374-1-AP | Download protocol |
| IHC protocol for RBM17 antibody 15374-1-AP | Download protocol |
| IP protocol for RBM17 antibody 15374-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD. |