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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM17 Recombinant antibody, PBS Only

RBM17 Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230125A10

Cat no : 82889-2-PBS

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of MCF-7 cells using RBM17 Recombinant antibody (82889-2-RR)

    IP experiment of MCF-7 using 82889-2-RR (same clone as 82889-2-PBS)

    IP result of anti-RBM17 (IP:82889-2-RR, 4ug; Detection:82889-2-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RBM17 Recombinant antibody (82889-2-RR)

    IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RBM17 Recombinant antibody (82889-2-RR)

    IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM17 Recombinant antibody (82889-2-RR)

    IF Staining of HeLa using 82889-2-RR (same clone as 82889-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM17 antibody (82889-2-RR, Clone: 230125A10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using RBM17 Recombinant antibody (82889-2-RR)

    FC experiment of A431 using 82889-2-RR (same clone as 82889-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human RBM17 (82889-2-RR, Clone:230125A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82889-2-RR

    Affinity and Kinetic Characterization of 82889-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-2-RR against Human RBM17 were performed. The affinity constant is 2.86 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82889-2-PBS targets RBM17 in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM17 fusion protein Ag0985 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 17
Calculated molecular weight 45 kDa
GenBank accession numberBC007871
Gene symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.