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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM17 Recombinant antibody (82889-3-RR)

    WB analysis using 82889-3-RR (same clone as 82889-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82889-3-RR (RBM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RBM17 Recombinant antibody (82889-3-RR)

    WB analysis of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    WB result of RBM17 antibody (82889-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RBM17 Recombinant antibody (82889-3-RR)

    IP experiment of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    IP result of anti-RBM17 (IP:82889-3-RR, 4ug; Detection:82889-3-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RBM17 Recombinant antibody (82889-3-RR)

    IHC staining of human ovarian cancer using 82889-3-RR (same clone as 82889-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82889-3-RR (RBM17 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82889-3-RR

    Affinity and Kinetic Characterization of 82889-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-3-RR against Human RBM17 were performed. The affinity constant is 0.828 nM.

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  • Featured Product
  • KD/KO Validated

RBM17 Recombinant antibody, PBS Only

RBM17 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82889-3-PBS
Clone No.230125A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82889-3-PBS targets RBM17 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM17 fusion protein Ag0985 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 17
Calculated molecular weight 45 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96I25
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.