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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM19 Polyclonal antibody

RBM19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19095-1-AP

Synonyms

KIAA0682, RBM19, RNA binding motif protein 19

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using RBM19 Polyclonal antibody (19095-1-AP)

    WB analysis of mouse colon using 19095-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using RBM19 Polyclonal antibody (19095-1-AP)

    WB analysis of COLO 320 using 19095-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using RBM19 Polyclonal antibody (19095-1-AP)

    WB analysis of mouse small intestine using 19095-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM19 Polyclonal antibody (19095-1-AP)

    IHC staining of human colon using 19095-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM19 Polyclonal antibody (19095-1-AP)

    IHC staining of human colon using 19095-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RBM19 Polyclonal antibody (19095-1-AP)

    IF Staining of MCF-7 using 19095-1-AP

    Immunofluorescent analysis of MCF-7 cells, using RBM19 antibody 19095-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse colon tissue, COLO 320 cells, mouse small intestine tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19095-1-AP is RBM19 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM19 fusion protein Ag13545 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 19
Calculated molecular weight 107 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006137
Gene symbol RBM19
Gene ID (NCBI) 9904
RRIDAB_10640906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM19, also named as KIAA0682, is a 960 amino acid protein, which belongs to the RRM MRD1 family. RBM19 is expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia. RBM19 plays a role in embryo pre-implantation development. The calculated molecular weight of RBM19 is 107 kDa, but the modified RBM19 is about 120 kDa.

Protocols

Product Specific Protocols
WB protocol for RBM19 antibody 19095-1-APDownload protocol
IHC protocol for RBM19 antibody 19095-1-APDownload protocol
IF protocol for RBM19 antibody 19095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols