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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM27 Polyclonal antibody (31850-1-AP)

    WB analysis using 31850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31850-1-AP (RBM27 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RBM27 Polyclonal antibody (31850-1-AP)

    IF Staining of U2OS using 31850-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM27 antibody (31850-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RBM27 Polyclonal antibody

RBM27 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31850-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RNA-Binding Protein 27, RNA Binding Motif Protein 27, KIAA1311

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Hela cell, jurkat cell, K-562 cell
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31850-1-AP targets RBM27 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM27 fusion protein Ag35822 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 27
Calculated molecular weight119 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_018989
Gene Symbol RBM27
Gene ID (NCBI) 54439
RRIDAB_3670129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9P2N5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM27 has been identified as a candidate autism gene, and is present in cytoplasmic and and nuclear speckles. RBM27 encodes a protein containing an RNA-Binding Motif, which confers its ability to bind RNA. Therefore, RBM27 is important in mRNA processing and the turnover of nuclear polyadenylate (pA+) RNA.

Protocols

Product Specific Protocols
WB protocol for RBM27 antibody 31850-1-APDownload protocol
IF protocol for RBM27 antibody 31850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols