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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM39 Monoclonal antibody (67420-1-Ig)

    WB analysis using 67420-1-Ig (same clone as 67420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM39 Monoclonal antibody (67420-1-Ig)

    WB analysis using 67420-1-Ig (same clone as 67420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RBM39 Monoclonal antibody (67420-1-Ig)

    IHC staining of human breast cancer using 67420-1-Ig (same clone as 67420-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67420-1-Ig (RBM39 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RBM39 Monoclonal antibody (67420-1-Ig)

    IHC staining of human breast cancer using 67420-1-Ig (same clone as 67420-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67420-1-Ig (RBM39 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM39 Monoclonal antibody (67420-1-Ig)

    IF Staining of HepG2 using 67420-1-Ig (same clone as 67420-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67420-1-Ig (RBM39 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM39 Monoclonal antibody (67420-1-Ig)

    IF Staining of HepG2 using 67420-1-Ig (same clone as 67420-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM39 antibody (67420-1-Ig, Clone: 2D2C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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RBM39 Monoclonal antibody, PBS Only

RBM39 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67420-1-PBS
Clone No.2D2C8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CAPER, CAPERalpha, CC1.3, DKFZp781C0423, FLJ44170, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67420-1-PBS targets RBM39 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RBM39 fusion protein Ag15766 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 39
Calculated molecular weight 530 aa, 59 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC141835
Gene Symbol RBM39
Gene ID (NCBI) 9584
RRIDAB_2882660
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14498
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.