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RBM4 Polyclonal antibody
RBM4 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, RIP, PLA, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11614-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human brain tissue, human heart tissue, human kidney tissue, HeLa cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human gliomas tissue, human lung cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
11614-1-AP targets RBM4 in WB, IP, IF, FC, RIP, PLA, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RBM4 fusion protein Ag2190 相同性解析による交差性が予測される生物種 |
Full Name | RNA binding motif protein 4 |
Calculated molecular weight | 40 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC021120 |
Gene symbol | RBM4 |
Gene ID (NCBI) | 5936 |
RRID | AB_2176095 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4
Protocols
Product Specific Protocols | |
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WB protocol for RBM4 antibody 11614-1-AP | Download protocol |
IHC protocol for RBM4 antibody 11614-1-AP | Download protocol |
IP protocol for RBM4 antibody 11614-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Cell The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.
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Nucleic Acids Res Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein. | ||
Nat Commun A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation. | ||