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RBM4 Monoclonal antibody
RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3H3F12
Cat no : 60292-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, fetal human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
Positive IHC detected in | human cervical cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
60292-1-Ig targets RBM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | RBM4 fusion protein Ag2190 相同性解析による交差性が予測される生物種 |
Full Name | RNA binding motif protein 4 |
Calculated molecular weight | 40 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC021120 |
Gene symbol | RBM4 |
Gene ID (NCBI) | 5936 |
RRID | AB_2881407 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.
Protocols
Product Specific Protocols | |
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WB protocol for RBM4 antibody 60292-1-Ig | Download protocol |
IHC protocol for RBM4 antibody 60292-1-Ig | Download protocol |
IF protocol for RBM4 antibody 60292-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |