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  • Featured Product
  • KD/KO Validated

RBM4 Monoclonal antibody

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H3F12

Cat no : 60292-1-Ig

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RBM4 Monoclonal antibody (60292-1-Ig)

    WB analysis of HeLa using 60292-1-Ig

    WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using RBM4 Monoclonal antibody (60292-1-Ig)

    WB analysis using 60292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using RBM4 Monoclonal antibody (60292-1-Ig)

    WB analysis of fetal human brain using 60292-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM4 Monoclonal antibody (60292-1-Ig)

    IHC staining of human cervical cancer using 60292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using RBM4 Monoclonal antibody (60292-1-Ig)

    IHC staining of human stomach using 60292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using RBM4 Monoclonal antibody (60292-1-Ig)

    IHC staining of human stomach using 60292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM4 Monoclonal antibody (60292-1-Ig)

    IHC staining of human cervical cancer using 60292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM4 Monoclonal antibody (60292-1-Ig)

    IF Staining of HeLa using 60292-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, fetal human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60292-1-Ig targets RBM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBM4 fusion protein Ag2190 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 4
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC021120
Gene symbol RBM4
Gene ID (NCBI) 5936
RRIDAB_2881407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

Protocols

Product Specific Protocols
WB protocol for RBM4 antibody 60292-1-IgDownload protocol
IHC protocol for RBM4 antibody 60292-1-IgDownload protocol
IF protocol for RBM4 antibody 60292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols