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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RBM4B Polyclonal antibody (15412-1-AP)

    WB analysis of HEK-293 using 15412-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15412-1-AP (RBM4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RBM4B Polyclonal antibody (15412-1-AP)

    IP experiment of HEK-293 using 15412-1-AP

    IP result of anti-RBM4B (IP:15412-1-AP, 3ug; Detection:15412-1-AP 1:300) with HEK-293 cells lysate 1000ug.

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RBM4B Polyclonal antibody

RBM4B Polyclonal Antibody for WB, IP, ELISA
Cat No. 15412-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

RBM30, RBM4B, RBM4L, RNA binding motif protein 30, RNA binding motif protein 4B, RNA binding protein 30, RNA binding protein 4B, ZCCHC15, ZCRB3B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15412-1-AP targets RBM4B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM4B fusion protein Ag7679 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 4B
Calculated molecular weight 40 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC003503
Gene Symbol RBM4B
Gene ID (NCBI) 83759
RRIDAB_2181210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ04
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RBM4B antibody 15412-1-APDownload protocol
IP protocol for RBM4B antibody 15412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols