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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM6 Polyclonal antibody, PBS Only

RBM6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14360-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM6 Polyclonal antibody (14360-1-AP)

    WB analysis using 14360-1-AP (same clone as 14360-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using RBM6 Polyclonal antibody (14360-1-AP)

    WB analysis of mouse spleen using 14360-1-AP (same clone as 14360-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBM6 Polyclonal antibody (14360-1-AP)

    IP experiment of HeLa using 14360-1-AP (same clone as 14360-1-PBS)

    IP result of anti-RBM6 (IP:14360-1-AP, 4ug; Detection:14360-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RBM6 Polyclonal antibody (14360-1-AP)

    IHC staining of human pancreas cancer using 14360-1-AP (same clone as 14360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14360-1-AP (RBM6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM6 Polyclonal antibody (14360-1-AP)

    IF Staining of HeLa using 14360-1-AP (same clone as 14360-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14360-1-AP (RBM6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14360-1-PBS targets RBM6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM6 fusion protein Ag5743 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 6
Calculated molecular weight 129 kDa
Observed molecular weight 129 kDa
GenBank accession numberBC046643
Gene symbol RBM6
Gene ID (NCBI) 10180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBM6, also named as DEF3, is a 1123 amino acid protein, which may interact with FAM168B. RBM6 is expressed ubiquitously in adults tissues. RBM6 specifically binds poly(G) RNA homopolymers in vitro. RBM6 exists some isoforms with MV 129 kDa, 69 kDa, and 59 kDa. onversely, fetal thymus and adult kidney express very little RBM6. In the hemopoietic system of mice, the expression of RBM6 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development.