Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 21896-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21896-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21896-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21896-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 21896-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 21896-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 21896-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 21896-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 21896-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM7 antibody (21896-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RBM7 Polyclonal antibody

RBM7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21896-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells
Positive IHC detected in	human liver cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

21896-1-AP targets RBM7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RBM7 fusion protein Ag11106 相同性解析による交差性が予測される生物種
Full Name	RNA binding motif protein 7
Calculated molecular weight	266 aa, 31 kDa
Observed molecular weight	30-35 kDa
GenBank accession number	BC034381
Gene Symbol	RBM7
Gene ID (NCBI)	10179
RRID	AB_2878938
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y580
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).

Protocols

Product Specific Protocols
WB protocol for RBM7 antibody 21896-1-AP	Download protocol
IHC protocol for RBM7 antibody 21896-1-AP	Download protocol
IF protocol for RBM7 antibody 21896-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nat Commun Temporal-iCLIP captures co-transcriptional RNA-protein interactions Authors - Ross A Cordiner View Article
human	WB	Mol Cell ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts Authors - Jérôme O Rouvière View Article
human	WB	Mol Cell P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress. Authors - Andrii Bugai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 21896-1-AP

WB analysis of HeLa using 21896-1-AP