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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM7 Polyclonal antibody

RBM7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21896-1-AP

Synonyms

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HepG2 using 21896-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HeLa using 21896-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HepG2 using 21896-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HeLa using 21896-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human liver cancer using 21896-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human lung cancer using 21896-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human liver cancer using 21896-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human lung cancer using 21896-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM7 Polyclonal antibody (21896-1-AP)

    IF Staining of HepG2 using 21896-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21896-1-AP (RBM7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21896-1-AP targets RBM7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM7 fusion protein Ag11106 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 7
Calculated molecular weight 266 aa, 31 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC034381
Gene symbol RBM7
Gene ID (NCBI) 10179
RRIDAB_2878938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).

Protocols

Product Specific Protocols
WB protocol for RBM7 antibody 21896-1-APDownload protocol
IHC protocol for RBM7 antibody 21896-1-APDownload protocol
IF protocol for RBM7 antibody 21896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Temporal-iCLIP captures co-transcriptional RNA-protein interactions

Authors - Ross A Cordiner
View Article
humanWB

Mol Cell

ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts

Authors - Jérôme O Rouvière
View Article
humanWB

Mol Cell

P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.

Authors - Andrii Bugai
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect RBM7 with Western blot in HEK293T WT cell lysate, which worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T WT
RBM7 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:21896-1-AP)
FH

Roy (Verified Customer) (06-15-2024)

Works well to detect the NEXT complex subunit RBM7 from HeLa extracts (1/1000 Dilution, overnight Incubation at 4°C).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa