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  • Featured Product
  • KD/KO Validated

RBM8A,Y14 Polyclonal antibody

RBM8A,Y14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14958-1-AP

Synonyms

RBM8A, BOV-1, BOV 1C, BOV 1B, BOV 1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis using 14958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of HeLa using 14958-1-AP

    WB result of RBM8A,Y14 antibody (14958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of HeLa using 14958-1-AP

    WB result of RBM8A,Y14 antibody (14958-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis using 14958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of HeLa using 14958-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of Jurkat cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of Jurkat using 14958-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IP experiment of HeLa using 14958-1-AP

    IP result of anti-RBM8A,Y14 (IP:14958-1-AP, 4ug; Detection:14958-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human brain using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human spleen using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human spleen using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human ovary using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human ovary using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human testis using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human testis using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human brain using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IF Staining of HeLa using 14958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IF Staining of HeLa using 14958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IF Staining of HeLa using 14958-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells, MDA-MB-453s cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human spleen tissue, human testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14958-1-AP targets RBM8A,Y14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM8A,Y14 fusion protein Ag6800 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 8A
Calculated molecular weight 20 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC017088
Gene symbol RBM8A
Gene ID (NCBI) 9939
RRIDAB_2178820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IHC protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IF protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IP protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
View Article
mouseIF

RNA

An atlas of chromatoid body components.

Authors - Oliver Meikar
View Article
humanWB,IHC

Front Oncol

RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.

Authors - Yan Lin
  • KD Validated
View Article
humanWB

Cell Death Discov

CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.

Authors - Xiaojin Song
View Article
humanWB,IF

Cell Rep

The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

Authors - Fernando C Alsina
View Article