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  • Featured Product
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RBM8A,Y14 Monoclonal antibody

RBM8A,Y14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F5G1

Cat no : 67541-1-Ig

Synonyms

RBM8A, BOV 1C, BOV 1B, BOV 1A, BOV 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM8A,Y14 Monoclonal antibody (67541-1-Ig)

    WB analysis using 67541-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using RBM8A,Y14 Monoclonal antibody (67541-1-Ig)

    WB analysis using 67541-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM8A,Y14 Monoclonal antibody (67541-1-Ig)

    WB analysis of HeLa using 67541-1-Ig

    WB result of RBM8A,Y14 antibody (67541-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RBM8A,Y14 Monoclonal antibody (67541-1-Ig)

    IHC staining of human ovary tumor using 67541-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RBM8A,Y14 Monoclonal antibody (67541-1-Ig)

    IHC staining of human ovary tumor using 67541-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RBM8A,Y14 Monoclonal antibody (67541-1-Ig)

    IF Staining of A549 using 67541-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM8A,Y14 antibody (67541-1-Ig, Clone: 1F5G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67541-1-Ig targets RBM8A,Y14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBM8A,Y14 fusion protein Ag6800 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 8A
Calculated molecular weight 20 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC017088
Gene symbol RBM8A
Gene ID (NCBI) 9939
RRIDAB_2882760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RBM8A,Y14 antibody 67541-1-IgDownload protocol
IHC protocol for RBM8A,Y14 antibody 67541-1-IgDownload protocol
IF protocol for RBM8A,Y14 antibody 67541-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity

Authors - Dan Li
View Article