Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells, HEK-293 cells, Jurkat cells, NCCIT cells, HeLa cells, Neuro-2a cells |
| Positive IP detected in | HepG2 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human placenta tissue, mouse brain tissue, human ovary tumor tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
12498-1-AP targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FOX2/RBM9 fusion protein Ag3168 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 9 |
| Calculated molecular weight | 390 aa, 41 kDa |
| Observed molecular weight | 50 and 60 kDa |
| GenBank accession number | BC013115 |
| Gene Symbol | RBM9 |
| Gene ID (NCBI) | 23543 |
| RRID | AB_2877861 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43251 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IHC protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IF protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IP protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Integrated pan-cancer analysis reveals the immunological and prognostic potential of RBFOX2 in human tumors | ||
Nat Cell Biol RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation | ||
iScience Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis |