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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBMS1 Recombinant antibody

RBMS1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240677D6

Cat no : 83623-5-RR

Synonyms

MSSP 2, MSSP 1, MSSP, HCC 4, C2orf12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBMS1 Recombinant antibody (83623-5-RR)

    WB analysis using 83623-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83623-5-RR (RBMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using RBMS1 Recombinant antibody (83623-5-RR)

    FC experiment of HepG2 using 83623-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBMS1 Recombinant antibody (83623-5-RR, Clone:240677D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83623-5-RR

    Affinity and Kinetic Characterization of 83623-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83623-5-RR against Human RBMS1 were performed. The affinity constant is 0.726 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, DU145 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83623-5-RR is RBMS1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBMS1 fusion protein Ag1493 相同性解析による交差性が予測される生物種
Full Name RNA binding motif, single stranded interacting protein 1
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC018951
Gene symbol RBMS1
Gene ID (NCBI) 5937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBMS1 (RNA binding motif single stranded interacting protein 1), also known as C2orf12. It is expected to be located in the nucleus, which is ubiquitinated in placenta and endometrium. Highest amounts are found in placenta, lung and heart. This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The molecular weight of RBMS1 is 45 kDa.

Protocols

Product Specific Protocols
WB protocol for RBMS1 antibody 83623-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols