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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RBP4 Recombinant antibody (84496-1-RR)

    WB analysis of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84496-1-RR (RBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01338-1

    Sandwich ELISA standard curve of MP01338-1

    Sandwich ELISA standard curve of MP01338-1, Human RBP4 Recombinant Matched Antibody Pair - PBS only. 84496-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31642. 84496-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    Urine of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 90.68 ng/mL with a range of 29.96 - 290.83 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    Saliva of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 12.14 ng/mL with a range of 8.34-17.17 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human RBP4, and measured 707.54 ng/mL.

  • Sample test of MP01338-1

    Sample test of MP01338-1

    Serum of twelve individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 87,804.85 ng/mL with a range of 37,160.99 - 156,178.64 ng/mL.

  • Flow cytometry (FC) experiment of HepG2 cells using RBP4 Recombinant antibody (84496-1-RR)

    FC experiment of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBP4 Recombinant antibody (84496-1-RR, Clone:241944B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01338-1

    Cytometric bead array standard curve of MP01338-1

    Cytometric bead array standard curve of MP01338-1, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-2-PBS. Standard: Eg31642. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01338-2

    Cytometric bead array standard curve of MP01338-2

    Cytometric bead array standard curve of MP01338-2, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-4-PBS. Standard: Eg31642. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01338-3

    Cytometric bead array standard curve of MP01338-3

    Cytometric bead array standard curve of MP01338-3, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-3-PBS. Standard: Eg31642. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84496-1-RR

    Affinity and Kinetic Characterization of 84496-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84496-1-RR against Human RBP4 were performed. The affinity constant is 0.504 nM.

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RBP4 Recombinant antibody, PBS Only (Capture)

RBP4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84496-1-PBS
Clone No.241944B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84496-1-PBS targets RBP4 as part of a matched antibody pair:

MP01338-1: 84496-1-PBS capture and 84496-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01338-2: 84496-1-PBS capture and 84496-4-PBS detection (validated in Cytometric bead array)

MP01338-3: 84496-1-PBS capture and 84496-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name retinol binding protein 4, plasma
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight20-21 kDa
GenBank accession numberBC020633
Gene Symbol RBP4
Gene ID (NCBI) 5950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02753
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)