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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBP4 Recombinant antibody

RBP4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241944H4

Cat no : 84496-4-RR

Synonyms

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBP4 Recombinant antibody (84496-4-RR)

    WB analysis using 84496-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84496-4-RR (RBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84496-4-RR

    Affinity and Kinetic Characterization of 84496-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84496-4-RR against Human RBP4 were performed. The affinity constant is 32.4 nM.

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Tested Applications

Positive WB detected inHepG2 cells, human plasma，human liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84496-4-RR targets RBP4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name retinol binding protein 4, plasma
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC020633
Gene symbol RBP4
Gene ID (NCBI) 5950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

Protocols

Product Specific Protocols
WB protocol for RBP4 antibody 84496-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols