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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBPJ Polyclonal antibody

RBPJ Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30044-1-AP

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using RBPJ Polyclonal antibody (30044-1-AP)

    WB analysis of MCF-7 using 30044-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RBPJ Polyclonal antibody (30044-1-AP)

    WB analysis of HepG2 using 30044-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RBPJ Polyclonal antibody (30044-1-AP)

    WB analysis of NIH/3T3 using 30044-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using RBPJ Polyclonal antibody (30044-1-AP)

    IP experiment of HepG2 using 30044-1-AP

    IP result of anti-RBPJ (IP:30044-1-AP, 4ug; Detection:30044-1-AP 1:1000) with HepG2 cells lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RBPJ Polyclonal antibody (30044-1-AP)

    IHC staining of human pancreas cancer using 30044-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RBPJ Polyclonal antibody (30044-1-AP)

    IHC staining of human pancreas cancer using 30044-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30044-1-AP targets RBPJ in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBPJ fusion protein Ag32552 相同性解析による交差性が予測される生物種
Full Name recombination signal binding protein for immunoglobulin kappa J region
Calculated molecular weight 56 kDa
Observed molecular weight~60 kDa
GenBank accession numberBC064976
Gene symbol RBPJ
Gene ID (NCBI) 3516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.

Protocols

Product Specific Protocols
WB protocol for RBPJ antibody 30044-1-APDownload protocol
IHC protocol for RBPJ antibody 30044-1-APDownload protocol
IP protocol for RBPJ antibody 30044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols