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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBX1 Monoclonal antibody, PBS Only

RBX1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4D9

Cat no : 66716-1-PBS

Synonyms

BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBX1 Monoclonal antibody (66716-1-Ig)

    WB analysis using 66716-1-Ig (same clone as 66716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using RBX1 Monoclonal antibody (66716-1-Ig)

    WB analysis of RAW 264.7 using 66716-1-Ig (same clone as 66716-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBX1 Monoclonal antibody (66716-1-Ig)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBX1 Monoclonal antibody (66716-1-Ig)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66716-1-PBS targets RBX1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBX1 fusion protein Ag7005 相同性解析による交差性が予測される生物種
Full Name ring-box 1
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC001466
Gene symbol RBX1
Gene ID (NCBI) 9978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.