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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBX1 Monoclonal antibody (66716-1-Ig)

    WB analysis using 66716-1-Ig (same clone as 66716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using RBX1 Monoclonal antibody (66716-1-Ig)

    WB analysis of RAW 264.7 using 66716-1-Ig (same clone as 66716-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBX1 Monoclonal antibody (66716-1-Ig)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBX1 Monoclonal antibody (66716-1-Ig)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBX1 Monoclonal antibody (66716-1-Ig)

    IF Staining of A431 using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBX1 antibody (66716-1-Ig, Clone: 1F4D9 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

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RBX1 Monoclonal antibody, PBS Only

RBX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66716-1-PBS
Clone No.1F4D9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein ZYP, EC:2.3.2.27, E3 ubiquitin-protein transferase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein ligase RBX1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66716-1-PBS targets RBX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBX1 fusion protein Ag7005 相同性解析による交差性が予測される生物種
Full Name ring-box 1
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC001466
Gene Symbol RBX1
Gene ID (NCBI) 9978
RRIDAB_2882067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP62877
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.