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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RC3H1 Polyclonal antibody (31234-1-AP)

    WB analysis using 31234-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31234-1-AP (RC3H1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RC3H1 Polyclonal antibody (31234-1-AP)

    IHC staining of human ovary cancer using 31234-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RC3H1 Polyclonal antibody (31234-1-AP)

    IHC staining of human ovary cancer using 31234-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RC3H1 Polyclonal antibody (31234-1-AP)

    IF Staining of HeLa using 31234-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RC3H1 antibody (31234-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RC3H1 Polyclonal antibody

RC3H1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31234-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ROQUIN, RNF198, RING finger protein 198, KIAA2025

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in4T1 cells, HEK-293T cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31234-1-AP targets RC3H1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RC3H1 fusion protein Ag35089 相同性解析による交差性が予測される生物種
Full Name ring finger and CCCH-type zinc finger domains 1
Calculated molecular weight126 kDa
Observed molecular weight130-150 kDa
GenBank accession numberNM_001300850
Gene Symbol RC3H1
Gene ID (NCBI) 149041
RRIDAB_3669912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5TC82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)

Protocols

Product Specific Protocols
WB protocol for RC3H1 antibody 31234-1-APDownload protocol
IHC protocol for RC3H1 antibody 31234-1-APDownload protocol
IF protocol for RC3H1 antibody 31234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols