Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 14869-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (Calcipressin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 cells using 14869-1-AP
WB result of RCAN1 antibody (14869-1-AP, 1:1000) with si-control and si-RCAN1 transfected SKOV-3 cells.
WB analysis of SKOV-3 using 14869-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14869-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14869-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14869-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14869-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 14869-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (RCAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14869-1-AP
IP result of anti-Calcipressin 1 (IP:14869-1-AP, 4ug; Detection:14869-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of human colon cancer using 14869-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14869-1-AP (RCAN1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 14869-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14869-1-AP (RCAN1 Antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

Calcipressin 1 Polyclonal antibody

Calcipressin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14869-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

ADAPT78, Calcipressin 1, CSP1, DSC1, DSCR1, MCIP1, RCAN1, RCN1, regulator of calcineurin 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, SKOV-3 cells, human heart tissue, mouse skeletal muscle tissue, mouse heart tissue, A431 cells, mouse cerebellum tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

14869-1-AP targets Calcipressin 1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Calcipressin 1 fusion protein Ag6457 相同性解析による交差性が予測される生物種
Full Name	regulator of calcineurin 1
Calculated molecular weight	28 kDa
Observed molecular weight	25-29 kDa
GenBank accession number	BC002864
Gene Symbol	RCAN1
Gene ID (NCBI)	1827
RRID	AB_10640904
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P53805
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Calcipressin 1 antibody 14869-1-AP	Download protocol
IHC protocol for Calcipressin 1 antibody 14869-1-AP	Download protocol
IP protocol for Calcipressin 1 antibody 14869-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med N6-methyladenosine modification of SPOP relieves ferroptosis and diabetic cardiomyopathy by enhancing ubiquitination of VDAC3 Authors - Wei Meng View Article
human	WB	Front Med (Lausanne) The identification of hub-methylated differentially expressed genes in osteoarthritis patients is based on epigenomic and transcriptomic data Authors - Zhen-Chen Chu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14869-1-AP

WB analysis of SKOV-3 cells using 14869-1-AP

WB analysis of SKOV-3 using 14869-1-AP

WB analysis of human heart using 14869-1-AP

WB analysis of mouse skeletal muscle using 14869-1-AP

WB analysis of mouse heart using 14869-1-AP

WB analysis of A431 using 14869-1-AP

WB analysis of mouse cerebellum using 14869-1-AP

IP experiment of mouse brain using 14869-1-AP

IHC staining of human colon cancer using 14869-1-AP