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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCAN2 Polyclonal antibody (12900-1-AP)

    WB analysis using 12900-1-AP (same clone as 12900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IP experiment of mouse brain using 12900-1-AP (same clone as 12900-1-PBS)

    IP result of anti-RCAN2 (IP:12900-1-AP, 4ug; Detection:12900-1-AP 1:300) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human gliomas using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human gliomas using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human skin using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human skin using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RCAN2 Polyclonal antibody, PBS Only

RCAN2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12900-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12900-1-PBS targets RCAN2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCAN2 fusion protein Ag3597 相同性解析による交差性が予測される生物種
Full Name regulator of calcineurin 2
Calculated molecular weight 225 aa, 25 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC038509
Gene Symbol RCAN2
Gene ID (NCBI) 10231
RRIDAB_10638913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14206
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RCAN2 is a member of the regulator of calcineurin (RCAN) protein family. These proteins play a role in many physiological processes by binding to the catalytic domain of calcineurin A, inhibiting calcineurin-mediated nuclear translocation of the transcription factor NFATC1. Expression of this gene in skin fibroblasts is upregulated by thyroid hormone, and the encoded protein may also play a role in endothelial cell function and angiogenesis.