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  • Featured Product
  • KD/KO Validated

RCAN2 Polyclonal antibody, PBS Only

RCAN2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12900-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCAN2 Polyclonal antibody (12900-1-AP)

    WB analysis using 12900-1-AP (same clone as 12900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IP experiment of mouse brain using 12900-1-AP (same clone as 12900-1-PBS)

    IP result of anti-RCAN2 (IP:12900-1-AP, 4ug; Detection:12900-1-AP 1:300) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human gliomas using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human gliomas using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human skin using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using RCAN2 Polyclonal antibody (12900-1-AP)

    IHC staining of human skin using 12900-1-AP (same clone as 12900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12900-1-PBS targets RCAN2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCAN2 fusion protein Ag3597 相同性解析による交差性が予測される生物種
Full Name regulator of calcineurin 2
Calculated molecular weight 225 aa, 25 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC038509
Gene symbol RCAN2
Gene ID (NCBI) 10231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RCAN2 is a member of the regulator of calcineurin (RCAN) protein family. These proteins play a role in many physiological processes by binding to the catalytic domain of calcineurin A, inhibiting calcineurin-mediated nuclear translocation of the transcription factor NFATC1. Expression of this gene in skin fibroblasts is upregulated by thyroid hormone, and the encoded protein may also play a role in endothelial cell function and angiogenesis.