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Species Reactivity

Species Reactivity

Conjugate

Conjugate

RCAS1 Monoclonal antibody, PBS Only

RCAS1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H8A12

Cat no : 66170-1-PBS

Synonyms

EB9, EBAG9, PDAF, RCAS1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RCAS1 Monoclonal antibody (66170-1-Ig)

    WB analysis of HEK-293 using 66170-1-Ig (same clone as 66170-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66170-1-Ig (RCAS1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCAS1 Monoclonal antibody (66170-1-Ig)

    IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCAS1 Monoclonal antibody (66170-1-Ig)

    IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCAS1 Monoclonal antibody (66170-1-Ig)

    IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCAS1 Monoclonal antibody (66170-1-Ig)

    IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RCAS1 Monoclonal antibody (66170-1-Ig)

    IHC staining of human lung cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RCAS1 Monoclonal antibody (66170-1-Ig)

    IHC staining of human lung cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using RCAS1 Monoclonal antibody (66170-1-Ig)

    IF Staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RCAS1 antibody (66170-1-Ig, Clone: 4H8A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RCAS1 Monoclonal antibody (66170-1-Ig)

    IF Staining of MCF-7 using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RCAS1 antibody (66170-1-Ig, Clone: 4H8A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66170-1-PBS targets RCAS1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RCAS1 fusion protein Ag2905 相同性解析による交差性が予測される生物種
Full Name estrogen receptor binding site associated, antigen, 9
Calculated molecular weight 213 aa, 24 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC017729
Gene symbol EBAG9
Gene ID (NCBI) 9166
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.