RCC1 Polyclonal antibody

Name: RCC1 Polyclonal antibody
Brand: Proteintech
SKU: 22142-1-AP

RCC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22142-1-AP

Synonyms

RCC1 I, CHC1

Validation Data Gallery

Filter:

AllWBIPIHCIF-PIF/ICC

WB analysis using 22142-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22142-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 22142-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 22142-1-AP
IP result of anti-RCC1 (IP:22142-1-AP, 4ug; Detection:22142-1-AP 1:8000) with HEK-293 cells lysate 1360 ug.
IHC staining of human stomach using 22142-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 22142-1-AP (RCC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 22142-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 22142-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse brain using 22142-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 22142-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 22142-1-AP
Immunofluorescent analysis of A431 cells using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 22142-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, SH-SY5Y cells, COS-7 cells, HeLa cells, MCF-7 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human stomach tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

22142-1-AP targets RCC1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity	human, mouse, monkey
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RCC1 fusion protein Ag17569 相同性解析による交差性が予測される生物種
Full Name	regulator of chromosome condensation 1
Calculated molecular weight	421 aa, 45 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC010067
Gene symbol	RCC1
Gene ID (NCBI)	1104
RRID	AB_11183045
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1.

Protocols

Product Specific Protocols
WB protocol for RCC1 antibody 22142-1-AP	Download protocol
IHC protocol for RCC1 antibody 22142-1-AP	Download protocol
IF protocol for RCC1 antibody 22142-1-AP	Download protocol
IP protocol for RCC1 antibody 22142-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Curr Biol Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development. Authors - Diana Vargas-Hurtado View Article
mouse	WB	Biomaterials Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells. Authors - Yudong Song View Article
human	WB	J Med Chem Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies. Authors - Brianna D Mackie View Article
human	IHC	Cell Death Dis Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells. Authors - Lijun Qiao View Article
mouse	WB	Exp Gerontol Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle. Authors - Shama R Iyer View Article
human	WB	J Cell Mol Med SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing Authors - Xi Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 22142-1-AP

WB analysis of HEK-293 using 22142-1-AP

WB analysis of NIH/3T3 using 22142-1-AP

IP experiment of HEK-293 using 22142-1-AP

IHC staining of human stomach using 22142-1-AP

IHC staining of human small intestine using 22142-1-AP

IHC staining of human small intestine using 22142-1-AP

IF Staining of mouse brain using 22142-1-AP

IF Staining of mouse brain using 22142-1-AP

IF Staining of A431 using 22142-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development.

Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.

Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.

Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells.

Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.

SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing