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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RCL1 Monoclonal antibody

RCL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D1B3

Cat no : 68221-1-Ig

Synonyms

RCL1, RNAC, RPC2, RPCL1, RTC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCL1 Monoclonal antibody (68221-1-Ig)

    WB analysis using 68221-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68221-1-Ig (RCL1 antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68221-1-Ig targets RCL1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RCL1 fusion protein Ag7058 相同性解析による交差性が予測される生物種
Full Name RNA terminal phosphate cyclase-like 1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC001025
Gene symbol RCL1
Gene ID (NCBI) 10171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA 3'-terminal phosphate cyclase-like protein (RCL1) does not have cyclase activity. It plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. RCL1 has two isoforms with the molecular weight of 41 kDa and 20 kDa.

Protocols

Product Specific Protocols
WB protocol for RCL1 antibody 68221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols