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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCN1 Recombinant antibody (83638-3-RR)

    WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RCN1 Recombinant antibody (83638-3-RR)

    WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RCN1 Recombinant antibody (83638-3-RR)

    IF Staining of U2OS using 83638-3-RR (same clone as 83638-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RCN1 antibody (83638-3-RR, Clone: 240473E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RCN1 Recombinant antibody (83638-3-RR)

    FC experiment of HeLa using 83638-3-RR (same clone as 83638-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RCN1 Recombinant antibody (83638-3-RR, Clone:240473E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00637-3

    Cytometric bead array standard curve of MP00637-3

    Cytometric bead array standard curve of MP00637-3, RCN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83638-4-PBS. Detection antibody: 83638-3-PBS. Standard: Ag13256. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83638-3-PBS

    Affinity and Kinetic Characterization of 83638-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83638-3-PBS against Human RCN1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83638-3-RR

    Affinity and Kinetic Characterization of 83638-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83638-3-RR against Human RCN1 were performed. The affinity constant is below 1 pM.

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RCN1 Recombinant antibody, PBS Only (Detector)

RCN1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83638-3-PBS
Clone No.240473E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Reticulocalbin 1, Reticulocalbin-1, RCN, RCAL, PIG20

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83638-3-PBS targets RCN1 as part of a matched antibody pair:

MP00637-3: 83638-4-PBS capture and 83638-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RCN1 fusion protein Ag13256 相同性解析による交差性が予測される生物種
Full Name reticulocalbin 1, EF-hand calcium binding domain
Calculated molecular weight 39 kDa
Observed molecular weight 44-46 kDa
GenBank accession numberBC010120
Gene Symbol RCN1
Gene ID (NCBI) 5954
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15293
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Reticulocalbin-1 (RCN1) belongs to the CREC family. Reticulocalbin‐1, an endoplasmic reticulum protein, plays a crucial role in calcium homeostasis and inhibits ER stress‐induced apoptosis (PMID: 28319095). Its main functions include maintaining intracellular homeostasis and regulating cell proliferation and apoptosis, and it plays an important role in the development of various tumours (PMID: 38057406). RCN1 is expressed aberrantly and at a high level in various tumors, including acute myeloid leukemia (AML) (PMID: 37746742). In multiple cancers, such as glioblastoma, non‐small cell lung cancer, renal cell carcinoma, hepatocellular carcinoma, and oral squamous cell carcinoma, the overexpression of RCN1 has been observed indicating its involvement in tumorigenesis and invasion (PMID: 30172915, PMID: 34722631).