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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCN2 Recombinant antibody (84956-4-RR)

    WB analysis using 84956-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RCN2 Recombinant antibody (84956-4-RR)

    WB analysis using 84956-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RCN2 Recombinant antibody (84956-4-RR)

    IF Staining of HeLa using 84956-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RCN2 Recombinant antibody (84956-4-RR)

    IF Staining of HepG2 using 84956-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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RCN2 Recombinant antibody

RCN2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84956-4-RR
Clone No.242612A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55, Calcium binding protein ERC 55

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat liver tissue, HeLa cells, HEK-293 cells, U-251 cells, mouse liver tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84956-4-RR targets RCN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RCN2 fusion protein Ag0247 相同性解析による交差性が予測される生物種
Full Name reticulocalbin 2, EF-hand calcium binding domain
Calculated molecular weight 37 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004892
Gene Symbol RCN2
Gene ID (NCBI) 5955
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14257
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

Protocols

Product Specific Protocols
WB protocol for RCN2 antibody 84956-4-RRDownload protocol
IF protocol for RCN2 antibody 84956-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols